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Laboratoř genové exprese

Biotechnologický ústav AV ČR

Průmyslová 595

252 50 Vestec

Czechia

Tel.::+420 325 873 748

DIČ: CZ86652036

IČ: 86652036

VAT No.: CZ86652036

Institute of Biotechnology

Instruments

BioMark™ System

Fluidigm The BioMark™ The BioMark™ line of life science products are meeting the challenges of today’s high throughput research environment through integrated fluidic circuits known as dynamic arrays and digital arrays. These innovative products allow scientists to practice tried-and-true techniques, such as TaqMan® assays, while realizing a previously unachievable throughput. Researchers save on reagents, pipette tips, time, and on the upkeep of expensive liquid-handling robots. Far more data are generated compared to microwell chips, and with no pre-spotting required.
  • Light source: Xenon lamp with excitation filters
  • Detection: CCD camera
  • Excitation wavelenghts: 465-505 nm, 510-550 nm
  • Emission wavelenghts: 500-550 nm, 540-600 nm
  • Examples of supported probe types:
    • FAM-MGB
    • VIC-MGB
    • FAM-TAMRA
    • FAM-non fluorescent quencher
  • Applications:
    • Gene expression: Microarray validation, Single cell gene expression, Drug efficacy and safety
    • Digital PCR: Absolute quantitation, Mutation detection
    • Genotyping: SNP association, Single cell genotyping

AVISO CellCelector TM

ALS Cell Celector The AVISO CellCelector is a freely configurable tool for the automated transfer of single cells and cell colonies. The patented harvest process allows a gentle cell uptake directly from the culture plate without pre-treatment. Using this technology, highest survival rate and cell integrity are guaranteed.

CellCelector Video

QIAcube

Qiagen QIAcube The innovative QIAcube uses advanced technology to process QIAGEN spin columns, enabling seamless integration of automated, low-throughput sample prep into your laboratory workflow. No change of purification chemistry is required, assuring fast startup and immediate results. All steps in the purification procedure are fully automated – and up to 12 samples can be processed per run.

For fully automated sample prep using spin-column kits

  • Automation of trusted QIAGEN spin-column kits
  • Elimination of manual processing steps
  • Purification of DNA, RNA, or proteins
  • More free time with affordable automated processing
  • Standardized results and increased productivity

Qiagen Video

2100 Bioanalyzer

Agilent Agilent The Agilent 2100 Bioanalyzer is a microfluidics-based platform for sizing, quantification and quality control of DNA, RNA, proteins and cells on a single platform. Results are delivered within 30-40 minutes in automated, high quality digital data.
  • RNA assays: RNA 6000 Nano total RNA kit, RNA 6000 Nano mRNA kit, RNA 6000 Pico total RNA kit, RNA 6000 Pico mRNA kit, Small RNA kit
  • DNA assays: DNA 1000 kit, DNA 7500 kit, DNA 12000 kit, High Sensitivity DNA kit
  • Protein assays: Protein 80 kit, Protein 230 kit, High Sensitivity Protein 250 kit

Experion Automated Electrophoresis Station

Bio-Rad Experion Automated Electrophoresis Station The Experion automated electrophoresis station performs all of the steps of gel-based electrophoresis in one compact, durable unit.It automates analysis by combining electrophoresis, staining, destaining, band detection, and imaging into a single, 30 minute step. It's an electrophoresis cell, power supply, and imager built into a single device.
  • RNA analysis:
    • RNA High sence chip for 11 samples, concentration range 100-5000 pg/ul, sample volume 1 ul
    • RNA Std sence chip for 12 samples, concentration range 5-500 ng/ul, sample volume 1 ul
  • DNA analysis:
    • DNA 1K assay chip for 11 samples, concentration range 0.1-50 ng/ul, separation range 15-1500 bp, sample volume 1 ul
    • DNA 12K assay chip for 11 samples, concentration range 0.1-50 ng/ul, separation range 50-17000 bp, sample volume 1 ul
  • Protein analysis:
    • P260 chip for 10 samples, concentration range 2.5-2000 ng/ul, separation range 100-260 kD, sample volume 4 ul

Experion Training Video: Chip Loading.

Bio-Rad CFX96 Real Time PCR Instrument

Bio-Rad Bio-Rad CFX96 Real Time PCR Instrument Bio-Rad’s next-generation PCR instruments meet all your PCR needs — whether you are designing your first experiment or running a high-throughput laboratory and analyzing large gene expression studies. The CFX96 real-time PCR detection system builds on the power and flexibility of the C1000™ thermal cycler, adding an easy-to-install interchangeable reaction module to create an exceptional real-time PCR system. The system’s unsurpassed thermal performance plus its innovative optical design produce accurate, reliable data, and the powerful yet intuitive software accelerates every step of your real-time PCR research, shortening the time between getting started and getting great results.
  • Light source: 6 LEDs in optics shuttle
  • Detection: 6 photodiodes
  • Excitation wavelenghts: 450–684 nm
  • Emission wavelenghts: 515–730 nm
  • Gradient range: 30–100°C, Maximum gradient span 24°C
  • Software for gene expression analysis
  • Precision melt software: Enables High Resolution Melt analysis, min. step for melt curve 0.2°C

Bio-Rad CFX384 Real Time PCR Instrument

Bio-Rad Bio-Rad CFX384 Real Time PCR Instrument Bio-Rad’s next-generation PCR instruments meet all your PCR needs — whether you are designing your first experiment or running a high-throughput laboratory and analyzing large gene expression studies. The CFX384 real-time PCR detection system builds on the power and flexibility of the C1000™ thermal cycler, adding an easy-to-install interchangeable reaction module to create an exceptional real-time PCR system. The system’s unsurpassed thermal performance plus its innovative optical design produce accurate, reliable data, and the powerful yet intuitive software accelerates every step of your real-time PCR research, shortening the time between getting started and getting great results.
  • Light source: 5 LEDs in optics shuttle
  • Detection: 5 photodiodes
  • Excitation wavelenghts: 450–690 nm
  • Emission wavelenghts: 515–730 nm
  • Gradient range: 30–100°C, Maximum gradient span 24°C
  • Software for gene expression analysis
  • Precision melt software: Enables High Resolution Melt analysis, min. step for melt curve 0.2°C

Bio-Rad C1000 Thermal Cycler

Bio-Rad Bio-Rad C1000 thermal cycler The C1000 thermal cycler offers superior performance in a fully modular platform able to accommodate different throughput needs with easily interchangeable reaction modules that swap in seconds without tools. Our module includes a gradient-enabled 96-well fast module. PCR module has a fully adjustable lid that supports a wide range of vessels and sealers for standard-height PCR plates.
  • Gradient temperature range 30-100°C
  • Gradient temperature differential 1-24°
  • Gradient accuracy ±0.2°C of programmed temperature at end rows

QX200 droplet digital PCR system

Bio-Rad Bio-Rad QX200 ddPCR systemThe QX200 Droplet Digital PCR (ddPCR™) System provides absolute quantification of target DNA or RNA molecules for EvaGreen or probe-based digital PCR applications.
  • Flexible digital PCR chemistry — optimized for TaqMan Hydrolysis Probe and EvaGreen Assays
  • Simple and easy-to-use workflow with 96-sample throughput
  • Droplet partitioning by the QX200 Droplet Digital technology reduces bias from amplification efficiency and PCR inhibitors
  • Convenient assay design — standard curves are not required
  • Measure varying degrees of cancer mutations, detect rare DNA target copies, and resolve copy number variation states with superior sensitivity and resolution

Eppendorf centrifuga 5804

Eppendorf Eppendorf centrifuga 5804 Eppendorf Model 5804R Multipurpose Centrifuges feature powerful, maintenance-free motors, and they accommodate a variety of rotors for unparalleled application versatility. They are three centrifuges in one: a high capacity general purpose centrifuge for cell harvesting; a high-speed centrifuge for separating cell lysates; and a micro centrifuge for DNA precipitations.Product features Interchangeable swing-bucket and high-speed fixed-angle rotors spin microcentrifuge tubes, popular glass and plastic tubes, microplates, and blood collection tubes Variable speed, maintenance-free motor spins up to 14,000 rpm, 20,800 x g Low profile 11-inch access height for easy sample loading Automatic rotor recognition senses rotor type to set maximum allowable speed-ensures safety.

Eppendorf centrifuga 5415R

Eppendorf Eppendorf centrifuga 5415R

Centrifuge 5415 R enables rapid and reliable refrigerated centrifugation in a temperature range between 0 °C and 40 °C. Temperature sensitive samples are maintained safely at 4 °C, even at maximumn speed. With the aid of the Fast Cool function, an almost chilly 4 °C can be attained within a mere 16 minutes. It has the indestructible maintenance-free drive, which requiresonly 13 seconds to bring the rotor to the maximum speed of 13,200 rpm (max. rcf: 16,110 x g).

Eppendorf epMotion P5073 Automated Pipetting System

Eppendorf Eppendorf R epMotion T 5070 Workstation The Eppendorf epMotion 5073 workstation enables fast and reproducible liquid handling by way of pipetting, dispensing, and other dispensing variants. With its great similarity to manual pipettes and to the familiar manual work method, pipetting sequences and protocols previously carried out manually can now be transferred quickly and easily to the epMotion 5073 workstation. Tried and true pipetting procedures have been maintained and standard techniques are quickly and easily transferred. Extensive experience with other liquid handling platforms and long training times are not needed.Valuable bench space is saved by using the integrated control panel instead of a PC. Operate the workstation using the keyboard of the connected control panel. The connected mouse enables free movement of the cursor over the integrated screen. Thus, an almost-intuitive operation with greater flexibility in the application is made possible. The user is led step by step through the software and prompted to carry out the necessary actions.

The following steps take place fully automatically following the insertion of the dispensing tool: the dispensing tool attaches the pipette tip; the liquid to be isolated is taken up from a supply and dispensed into one or more target tubes. Normally, the dispensing is concluded following the ejection of the pipette tip. Optical sensors control and ensure the reliability of the procedure:

  • Testing of liquid quantities
  • Testing of the existing pipette tip type with the programming
  • Testing of the existing tubes with the programming

The epMotion 5073 workstation enables quick filling of plates in the 384-well scale. It is also possible to fill an MTP 384-well from other formats (eg, 4 x MTP 96-well).

Listen to the Eppendorf song.

Qubit® 2.0 Fluorometer

Life Technologies Qubit The Qubit® 2.0 Fluorometer quantitates DNA, RNA, and protein with unprecedented accuracy, sensitivity, and simplicity. Qubit® fluorometric quantitation uses Qubit® assays that contain advanced dyes that only fluoresce when bound to DNA, RNA, or protein. This specificity allows you to get very accurate results because Qubit® technology only reports the concentration of the molecule of interest, not contaminants. Each Qubit® assay kit is highly sensitive for a single analyte. Samples with concentrations as low as 10 pg/uL of DNA, and 12.5 ug/mL of protein, may be accurately and reliably quantitated.

NanoDrop 1000

Thermo NanoDrop 1000 The Thermo Scientific NanoDropTM 1000 Spectrophotometer enables highly accurate UV/Vis analyses of 1 ul samples with remarkable reproducibility. The patented sample retention system eliminates the need for cuvettes and capillaries which decreases the measurement cycle time. In addition, the high absorbance capability eliminates the need for most dilutions. You’ll find yourself making fewer dilutions and taking more readings than ever before, essentially becoming a more quantitative laboratory.

The NanoDrop 1000 offers many benefits:

  • Small samples: designed for 1 ul samples
  • Large dynamic range: measures 2-3700 ng/ul (dsDNA) on a single sample. No need to change out cuvettes or caps.
  • High absorbance capability: 50 times that of traditional spectrophotometers
  • No Dilutions for most samples
  • No extra costs for consumables
  • Full Spectrum (220-750nm)
  • 10 second measurement time
  • No cuvettes or capillaries
  • Sample is recoverable
  • Small size (size of a tissue box)
  • Easy set up (plug and play)
  • Software designed for life sciences
  • Free software upgrades

You can watch ilustrative movie.

Rotor Gene 6000

Ilustration Ilustration

The Rotor-Gene 6000 is unlike any other real-time system. It uses a unique centrifugal rotary design, ideal for even the most demanding requirements of real-time thermo-optical analyses. What sets the Rotor-Gene apart is its amazing precision; the result of near-perfect well-to-well thermal and optical uniformity. Its design also enables a fast data acquisition rate, a capability now proving to be essential for advanced applications such as HRM (high-resolution melt).

  • Light source: Separate high intensity light-emitting diode (LED), excitation source for each channel
  • Detection: Photomultiplier (PMT) detector with variable or automatic gain setting (sensitivity control), user selected, separate emission filter for each channel
  • Excitation wavelenghts: 470±10 nm,530±5 nm, 460±15 (HRM)
  • Emission wavelenghts: 510±5 nm (green and HRM), 555±5 (yellow)
  • Uniformity of unique centrifugal rotary format: ± 0.01°C
  • 2-Plex + HRM (Green, Yellow) fluorescence channels
  • High resolution melt (HRM) application with smallest thermal increment 0.02°C
  • Holds 36 x 0.2 ml tubes, 72 x 0.1 ml strip tubes or 100 Gene-Disc
  • Software: Build-in extensive analysis, graphing and statistical functions. Unlimited use software license for Windows XP, Pentium IV (2GHz) or higher PC.
  • Examples of supported probe types:
    • Green: FAM, SYBR Green 1, Fluorescein, EvaGreen, Alexa Fluor 488, Chromofy
    • Yellow: JOE, VIC, HEX, TET, CAL Fluor Gold 540, Yakima Yellow
    • HRM: SYTO 9, LC Green, LC Green Plus+, EvaGreen, Chromofy

Watch a 3D animation of the Rotor-Gene 6000 via the You Tube.

StepOne Plus™ Real-Time PCR System

Ilustration Ilustration

The StepOne™ Real-Time PCR System delivers remarkable plug-and-play convenience and uncompromising performance. It supports the full range of TaqMan® assays while providing ease-of-use and a small footprint. Each system offers intuitive software, fast runs (< 40 minutes) and standard runs (< hours), and are configured for PC-free or networked operation. This factory-calibrated system makes it easy to step up to high performance real-time PCR.

  • Light source: Blue LED
  • Detection: Photodiodes
  • Examples of supported dye types: 4 filters factory calibrated for FAM/SYBR Green, VIC/JOE, NED/TAMRA and ROX dyes
  • Software for gene expression analysis

LightCycler 96 System

Ilustration Ilustration

The LightCycler® 96 Real-Time PCR System is a multiwell-plate based real-time PCR platform which is used for highly accurate qualitative and quantitative detection of nucleic acids and genotyping. An ideal combination of accuracy, temperature homogeneity, and reproducibility now enhanced by an interface so intuitive that is accessible to any user in the lab.

  • Light source: LED
  • Detection: CCD camera
  • Excitation and emission: Pairs of 96 robust fiber optic cables - half for the excitation, half for the emmision
  • Silver thermal block
  • 20°C gradient range
  • High Resolution Melting Analysis possible

LightCycler 480 System

Ilustration Ilustration

The LightCycler® 480 Real-Time PCR System is a multiwell-plate based real-time PCR platform which is used for highly accurate qualitative and quantitative detection of nucleic acids and genotyping. Building on the benefits of Roche's capillary-based LightCycler® 2.0 Systems, it goes one step further in offering enhanced throughput, compatibility with automation equipment and maximum flexibility regarding hard- and software.

  • Light source: Xenon lamp
  • Detection: CCD camera
  • Excitation filters: 440 nm, 465 nm, 498 nm, 533 nm, 618 nm
  • Emission filters: 488 nm, 510 nm, 580 nm, 610 nm, 640 nm, 660 nm
  • Silver thermal block
  • LightCycler 480 Software provides versatile solutions for the most common real-time applications
  • High Resolution Melting Analysis available

Eco Real-Time PCR System

Ilustration Ilustration

The Eco Real-Time PCR System combines true temperature control, four-color multiplex capabilities, an optimized optical system, and intuitive software to deliver highly accurate qPCR results. Its icon-driven user interface simplifies experimental design and setup, while a straight forward workflow streamlines operation to three easy steps: load samples into the 48-well plate, place the plate into the Eco instrument, and run.

  • Light source: LED
  • Detection: CCD camera
  • Excitation filters: 452–486 nm and 542–582 nm
  • Emission filters: 505–545 nm, 562–596 nm, 604–644 nm, and 665–705 nm
  • 48-well block, Single Peltier-based system
  • High Resolution Melting Analysis available

Quick Gene-Mini 80

Fujifilm Fujifilm

The key technology distinguishing the QuickGene systems from comparable products is Fujifilm’s patented 80 µm porous membrane, which selectively binds nucleic acids. This ultra-thin membrane with uniformly fine pores and a large specific binding surface ensures high yield and outstanding purity. The membrane’s unique features allow DNA extraction using air pressure, with no need for centrifugation.

  • Genomic DNA can be extracted manually from 8 samples simultaneously in just 12 minutes
  • The QuickGene systems can now be applied as CE IVD marked pre-analytic method for molecular diagnostics

GentleMACS™ Dissociator

MACS MACS

The gentleMACS™ Dissociator is a benchtop instrument for the automated dissociation of tissues. Two types of unique gentleMACS Tubes used with the instrument enable the time-saving and easy dissociation or homogenization of tissues in a closed system. C Tubes are used for the gentle preparation of single-cell suspensions from tissues, whereas M Tubes are used for the homogenization of tissues or cells. A single sample or two samples in parallel can be processed. The instrument offers optimized gentleMACS Programs for a variety of specific applications. The standardized tissue dissociation or homogenization procedures make for reliable and reproducible results.

VIAFLO Voyager adjusting multichanel pipette

MACS MACS

VIAFLO's Voyager electronic adjustable tip pipette makes multichannel sample transfers from tubes to plates or loading gels simple and efficient. Tip spacing can be changed from 4.5mm to 19.5mm simply by pressing a button.